Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

Maize is one of the most important crop species worldwide, but also extremely susceptible to effects increasingly higher temperatures and drought during summer its flowering grain filling stage. Different strategies are being utilized ensure a satisfying yield potential quality even in unfavourable environmental conditions, which result climate change. Some them cropping pattern changes sowing alterations, including earlier sowing. Since this implies exposure suboptimal early developmental stages, it leads demand for developing maize lines tolerant low these stages. This research focuses on first phase germination, imbibition. tolerance complex trait that includes different mechanisms all work together adaptation survival, such as cell membrane changes, antioxidative system activation, etc. Additionally, temperature response inbreds varies substantially, so recognizing ways they respond imbibition other stages development crucial. To accomplish this, seeds two inbred contrasting susceptibility were selected exposed control (20? C) (8?C) conditions 24h imbition then further analyzed assess their response. included germination tetrazolium assays; ascertaining integrity by evaluating leakage lipid peroxidation; determining capacity assessing superoxide dismutase (SOD) catalase (CAT) activity; expression analysis four genes (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid desaturases 2 6, fad2 fad6; plastid-lipid-associated protein, pap2). The results showed that, while there not significant difference rate, differ survival with more genotype surviving period. Significant differences between found (p<0,01), well gid1(p<0,05) fad6 (p<0,05) gene assays. present brings light our understanding effect stage, - It highlights importance choosing right points certain routes could be taken improving accelerating breeding process tolerance.